rabbit polyclonal anti transferrin receptor  (Thermo Fisher)

Journal: Journal of Nanobiotechnology

Article Title: Tungsten-based polyoxometalate nanoclusters as ferroptosis inhibitors modulating S100A8/A9-mediated iron metabolism pathway for managing intracerebral haemorrhage

doi: 10.1186/s12951-025-03149-9

Figure Lengend Snippet: W-POM NCs for managing intracerebral haemorrhage. Schematic illustration of W-POM NCs inhibiting ferroptosis by modulating the S100A8/A9-mediated iron metabolism pathway. The nanoclusters targeted oxidative stress and iron dysregulation to manage ICH, demonstrating their potential for neuroprotection and therapeutic efficacy. The figures were created using BioRender.com. ICH, intracerebral haemorrhage; W-POM NCs, tungsten-based polyoxometalate nanoclusters; TFR1, Transferrin Receptor 1; DMT1, Divalent Metal Transporter 1; FPN, Ferroportin; TLR4, Toll-like receptor 4; ROS, Reactive Oxygen Species

Article Snippet: The following primary antibodies were detected via WB: rabbit anti-transferrin receptor 1 (TFR1) polyclonal antibody (1:1000, AF5343, Affbiotech, China), rabbit anti-FPN polyclonal antibody (1:1000, NBP1-21502, Novus, US), rabbit anti-divalent metal transporter 1 (DMT1) polyclonal antibody (1:1000, 20507-1-AP, Proteintech, US), rabbit anti-S100A8/A9 monoclonal antibody (1:1000, ab288715, Abcam, UK), rabbit anti-hepcidin monoclonal antibody (1:200, ab190775, Abcam, UK), anti-TLR4 polyclonal antibody (1:1000, AF7017, Affbiotech, China).

Techniques: Drug discovery

Journal: Journal of Nanobiotechnology

Article Title: Tungsten-based polyoxometalate nanoclusters as ferroptosis inhibitors modulating S100A8/A9-mediated iron metabolism pathway for managing intracerebral haemorrhage

doi: 10.1186/s12951-025-03149-9

Figure Lengend Snippet: W-POM NCs inhibit ICH-induced neuroinflammation, oxidative stress and ferroptosis in ICH mice model. ( a ) Protein expression of iron transport-related proteins (TFR1, FPN, DMT1) assessed using western blot. ( b - d ) Statistical graph of grayscale values of TFR1, FPN, DMT1 protein expression. ( e ) Representative TEM images of mitochondrial morphology of the neurons in the perihaematomal area at 72 h after ICH. Red arrows indicate mitochondria. Representative images by Nissl staining ( f ) and FJB staining ( g ) in the perihaematomal tissue showing the effects of W-POM NCs on ICH-induced neuronal injury and neurodegeneration. Statistical analysis of Nissl staining ( h ) and FJB staining ( i ) results in the perihaematomal tissue. ( j ) Representative images of immunofluorescent staining for Iba-1 (red) and DAPI (blue) in the perihaematomal area at 72 h after ICH. ( l ) Quantitative analyses of Iba-1 positive cells in the perihaematomal area at 72 h after ICH. ( k ) Representative images of immunofluorescent staining for GFAP (red) and DAPI (blue) in the perihaematomal area at 72 h after ICH. ( m ) Quantitative analyses of GFAP-positive cells in the perihaematomal area at 72 h after ICH. (* P < 0.05, ** P < 0.01, *** P < 0.001, means ± SEM). ICH, intracerebral haemorrhage; TEM, transmission electron microscopy; W-POM NCs, tungsten-based polyoxometalate nanoclusters; TFR1, transferrin receptor 1; DMT1, divalent metal transporter 1; FPN, ferroportin

Article Snippet: The following primary antibodies were detected via WB: rabbit anti-transferrin receptor 1 (TFR1) polyclonal antibody (1:1000, AF5343, Affbiotech, China), rabbit anti-FPN polyclonal antibody (1:1000, NBP1-21502, Novus, US), rabbit anti-divalent metal transporter 1 (DMT1) polyclonal antibody (1:1000, 20507-1-AP, Proteintech, US), rabbit anti-S100A8/A9 monoclonal antibody (1:1000, ab288715, Abcam, UK), rabbit anti-hepcidin monoclonal antibody (1:200, ab190775, Abcam, UK), anti-TLR4 polyclonal antibody (1:1000, AF7017, Affbiotech, China).

Techniques: Expressing, Western Blot, Staining, Transmission Assay, Electron Microscopy

Journal: Neural Development

Article Title: Analysis of Lrrn1 expression and its relationship to neuromeric boundaries during chick neural development

doi: 10.1186/1749-8104-2-22

Figure Lengend Snippet: Endosomal distribution of Lrrn1. Immunocytochemical analysis of the endosomal uptake of FLAG-Lrrn1 in HeLa cells. (a, d, g, j) Plasma membrane surface-exposed FLAG-Lrrn1 protein detected with an anti-FLAG antibody. (b, c) Colocalisation with total cellular pool of Lrrn1 detected with the GST-IC antiserum. (e, f) Colocalisation with EEA1 (arrowheads in inset in (f)) shows that a significant pool of Lrrn1 cycles through the early endosomes. (h, i) Little overlap with transferrin receptor immunostaining suggests that Lrrn1 does not partition within recycling endosomes. (k-m) A very small proportion of endosomes were found to co-label with FLAG-Lrrn1/EGF ((m), arrowhead in inset). Scale bars = 200 μm except inset in (f) = 50μm.

Article Snippet: The antibody against EEA1 (rabbit polyclonal antiserum 1:1,500) was a kind gift of Dr Mario Zerial (EMBL, Heidelberg, Germany) and the anti-human transferrin receptor (rabbit polyclonal, 1:250) was from Chemicon.

Techniques: Immunostaining